An automated method for the determination of estrogens in pregnancy urine.
نویسندگان
چکیده
Ittrich’s method for the determination of total estrogens in urine (1) was adapted for automation on the AutoAnalyzer, using a continuous digester module for the combined operation of phase exchange and color development. The automated method gave good recovery and reproducibility, and correlated well with the manual method. It increased the capacity of this laboratory for estrogen determinations, improved the turnover rate, and reduced the labor involved to a minimum.
منابع مشابه
Automated hydrolysis of total estrogens in urine from pregnant women.
This method for the hydrolysis of estrogens completes the automated analysis of estrogens in pregnancy urine described by Muir, Ua Conaill, and Ryan [Steroids 13, 719 (1969)]. Pregnancy urines can be processed from the sampling of urine to the recording of fluorescence intensity in 30 mm, at a rate of 20 samples per hour. The presence of glucose does not de crease the apparent hydrolysis products.
متن کاملValidity of a simple automated method in which the "direct" Kober reaction is used to measure estrogens during pregnancy.
Using a simple automated procedure for measurement of total estrogens in urine, we found that several estrogen conjugates gave higher fluorescence yields than did the corresponding free steroids. In the analysis of urine samples from pregnant women, the “direct Kober reaction” gave consistently higher values than those obtained after preliminary acid or enzymatic hydrolysis. The effects of stor...
متن کاملA semi-automated fluorometric method for total estrogens in pregnancy urine.
We report a continuous-flow fluorometric method for total urinary estrogens that involves the Kober reaction, with extraction of the reaction product into dichloroethane containing trichloroacetic acid as described by Hahnel and Jones [Clin. Chim. Acta 16, 185 (1967)]. The dichloroethane extraction gives greater stability to the Kober color and sharper separation of aqueous and organic phases. ...
متن کاملUrinary estrogens in pregnancy; improved method for their determination in humans.
NE OF PEE DIFFICULTIES encountered in the determination of urinary estrogens arises from the fact that no procedure so far developed is capable of cleaving the estrogen conjugates quantitatively without destroying any of the free steroids (1). Reporting on a thorough investigation of this subject Van Bruggen (2) stated: “The use of butanol as the solvent and hydrolyzing medium.. . appears to be...
متن کاملارزیابی روش‌های الکتروایمونواسی و 100Clinitek- برای تشخیص میکروآلبومینوری
Evaluation of Electroimmunoassay and Clinitek-100 for Detection of Microalbuminuria B.A. Jalali Khanabadi PhD , H. Mozaffari Khosravi PhD Received: 12/10/05 Sent for Revision: 04/03/06 Received Revised Manuscript: 20/01/07 Accepted: 25/01/07 Background and Objective: Detection of microalbuminuria is an important laboratory test for evaluation of diabetic complications. A reliable me...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Clinical chemistry
دوره 14 10 شماره
صفحات -
تاریخ انتشار 1968